Fig. 1

qPCR analysis of liver subcellular fractions using select marker genes. Expression of each gene was determined by qPCR across the cytoplasm (C), nucleus (N), nucleoplasm (NP) and chromatin-bound (CB) fractions. Data shown are relative expression levels (values above each bar, with one of the bar set = 1.0 for each gene, as marked), which are presented as mean values + SEM for n = 4 mice per biological condition: vehicle treated male and female mice, and TCPOBOP-treated male and female mice. a Elovl3: male-biased expression seen in vehicle control group mice is largely lost following TCPOBOP treatment. b PreElovl3, assayed using qPCR primers that span an intron/exon boundary to amplify unspliced transcripts, which were significantly enriched in the chromatin-bound fraction after TCPOBOP exposure in both sexes. c Cyp2b10, validating TCPOBOP induction response, and also female-biased expression in the basal state. d Neat1 (lnc14746), highly chromatin-bound, validates the separation of nucleoplasmic and chromatin fractions. e The female-specific Xist (lnc15394), strong expression in all three nuclear-derived fractions. Significance was determined by one-way ANOVA with Bonferroni correction, for four separate analyses, which are specified using four different symbols (red box), as follows: p < 0.05, one symbol; p < 0.01, two symbols; p < 0.001, three symbols; and p < 0.0001, four symbols. qPCR primers are shown in Table S1A