Fig. 1

The validation of barley line Rolap plant material under low-Pi stress. a Pictures of the plants (n = 3) collected on the 23rd day after sowing, grown under low-Pi, 8 mg P/kg soil (left) and control-Pi, addition of 60 mg P/kg soil (right), conditions. b Shoot fresh tissue weight (n = 3). c The Pi concentration measurements performed for barley roots and shoots (n = 3). Asterisks indicate a significant difference (* p-value < 0.05) calculated using two-tailed Student’s t-tests. Scale bar = 10 cm. Error bars = SD