Fig. 2

The framework illustrating the data generation protocols used in this study. The low-Pi stress-specific subsets of RNAs were generated following (i) deep sequencing of small RNAs from barley shoots and roots, (ii) transcriptomic RNA-Seq for barley shoots, and (iii) degradome profiling for barley shoots and roots. The obtained data sets were mapped to the references collected from miRBase and Ensembl Plants databases. The log₂ scale for fold change and Bonferroni corrections were calculated to pick the significantly changed sequences under Pi-deficient and Pi-sufficient conditions