TY - JOUR AU - Rajewski, Alex AU - Carter-House, Derreck AU - Stajich, Jason AU - Litt, Amy PY - 2021 DA - 2021/03/22 TI - Datura genome reveals duplications of psychoactive alkaloid biosynthetic genes and high mutation rate following tissue culture JO - BMC Genomics SP - 201 VL - 22 IS - 1 AB - Datura stramonium (Jimsonweed) is a medicinally and pharmaceutically important plant in the nightshade family (Solanaceae) known for its production of various toxic, hallucinogenic, and therapeutic tropane alkaloids. Recently, we published a tissue-culture based transformation protocol for D. stramonium that enables more thorough functional genomics studies of this plant. However, the tissue culture process can lead to undesirable phenotypic and genomic consequences independent of the transgene used. Here, we have assembled and annotated a draft genome of D. stramonium with a focus on tropane alkaloid biosynthetic genes. We then use mRNA sequencing and genome resequencing of transformants to characterize changes following tissue culture. SN - 1471-2164 UR - https://doi.org/10.1186/s12864-021-07489-2 DO - 10.1186/s12864-021-07489-2 ID - Rajewski2021 ER -