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Table 1 Strain -and-sex dependent differences in body variables and glucose homeostasis

From: Strain-based and sex-biased differences in adrenal and pancreatic gene expression between KK/HlJ and C57BL/6 J mice

 

KK-M

KK-F

C57-M

C57-F

p-value strain*sex

 Body weight (g)

37.11** ± 0.58

36.99** ± 1.04

28.79$$ ± 0.35

21.34 ±0.21

< 0.001

 Visceral fat weight (g)

1.20** ± 0.03

3.78$$** ± 0.23

0.63$$ ± 0.04

0.27 ± 0.02

< 0.001

 Pancreas weight (g)

0.33* ±0.01

0.35* ± 0.05

0.30$$ ± 0.01

0.22 ± 0.01

0.100

Insulin Tolerance Test (ITT)

 Basal Glucose (mg/dL)

251.33$$$** ± 18.93

165.77** ± 5.50

136.14 ± 4.28

130.83 ± 3.52

< 0.001

 AUCGLUCOSE (mg/dL.min)

13,145.2$$$** ± 703.18

8790.41** ± 371.24

7404.58$$ ± 214.05

6346.80 ± 299.54

< 0.001

 KITT (%/min)

1.01 ± 0.11

1.07 ± 0.16

0.84 ± 0.07

1.22$$ ± 0.13

0.099

 T1/2GLUCOSE (min)

69.21 ± 8.24

69.65 ± 7.89

68.18$ ± 3.32

57.51 ± 6.31

0.720

Fasting Blood measurements

 Serum Glucose (mg/dL)

144.61 ± 5.28

144.42** ± 4.61

138.86$$$ ± 3.67

119.67 ± 2.52

0.024

 Serum Insulin (uIU/mL)

46.92** ± 4.71

32.16** ± 5.55

9.72 ± 0.54

8.51 ± 0.77

0.061

 HOMA-IR

16.30** ± 1.44

11.31** ± 1.90

3.32$ ± 0.19

2.50 ± 0.23

0.076

 C-Peptide

2134.30$$$** ± 281.96

975.47* ± 119.45

822.35$ ± 91.05

543.42 ± 96.07

0.007

 Glucagon (pg/ml)

29.02 ± 4.43

157.65*$$$ ± 16.63

35.15 ± 4.66

96.93$$$ ± 9.73

< 0.001

 Leptin

6360.02** ± 305.11

6070.94** ± 454.04

2439.69$$ ± 248.33

1291.04 ± 221.96

0.184

 Corticosterone (ng/ml)

103.36 ± 10.40

200.74$$$ ± 21.42

92.32 ± 9.56

160.67$$ ± 17.86

0.351

 Aldosterone (pg/ml)

649.63*** ± 50.60

571.13*** ± 45.26

329.75 ± 33.96

348.54 ± 31.15

< 0.0001

 IL-6 (pg/ml)

62.02 ± 13.20

82.44* ± 16.22

39.72 ± 4.64

31.08 ± 5.43

0.179

 TNF-α (pg/ml)

8.07* ± 0.96

9.25 ± 0.81

13.83$ ± 1.11

9.25 ± 1.56

0.015

  1. Data presented as means ± SEM, n=18 per group
  2. p-values for strain*sex were calculated using 2-way ANOVA
  3. A significance of p-value < 0.05, < 0.001 and < 0.0001 based on independent t-tests is indicated by *, ** and *** for significance between strains (C57-M vs KK-M and C57-F vs KK-F); and significant sex effect within each strain (i.e.C57-M vs C57-F and KK-M vs KK-F) is represented using $, $$ and $$$ for p < 0.05, < 0.01 and < 0.001 respectively
  4. AUCGLUCOSE Area Under the Curve for glucose, KITT Rate of glucose utilization, T1/2 Half-life of glucose levels, C57-M C57BL/6 J Males, C57-F C57BL/6 J Females, KK-M KK/HIJ Males, KK-F KK/HIJ Females
  5. Parts of data in Table 1 (rows 1–12, 16, 17) have been adapted from our related publication Inglis et al. (2019) [27]