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Fig. 3 | BMC Genomics

Fig. 3

From: Consistent ultra-long DNA sequencing with automated slow pipetting

Fig. 3

Comparison long read retention by pipetting methods. a. Distribution of log-transformed read lengths. Each violin plot represents the total reads sequenced across six library preparations. The Yellow region denotes 500–1000 kb. The orange region denotes 1–10 Mb b. Comparative read length histogram. Each point represents total base pairs sequenced across six libraries. The Y-axis denotes log-transformed total base pairs sequenced. The X-axis denotes length in base pairs. c. Averages of N50 values per pipetting method. Mean is denoted by center line. Standard deviation is denoted by upper and lower bounds. The Yellow region denotes 500–1000 kb, and the orange region reads greater than one Mb. d. Number of reads greater than 100 kb sequenced per flow cell. Significant difference is denoted by the asterisk (p-value = 0.0438). e. Total base pairs of reads greater than 100 kb sequenced per flow cell. Significant difference is denoted by the asterisk (p-value = 0.0415). f. Total base pairs of reads greater than 200 kb sequenced per flow cell. Significant difference is denoted by the asterisk (p-value = 0.0441)

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