Fig. 4

Bioinformatics analysis of Holotrichia parallela GST. a Schematic representation of H. parallela GST. The GST N-terminal and GST C-terminal are indicated in wathet blue and purple boxes, respectively. The G-site and H-site are shown above the boxes. b Sequence analysis of H. parallela GST. The GST N-terminal and GST C-terminal are indicated above the sequences. The GSH binding sites (G-site) are shown in red. The dimer interface (polypeptide binding site) is shown in dark grey. The C-terminal domain interface is underlined. The substrate binding pocket (H-site) is shown in purple. The N-terminal domain interface (polypeptide binding site) is underlined and tilted

c The overall structure of H. parallela GST. The N-terminus (N), C-terminus (C), α-helices and β-strands are labeled. ‘a’ and ‘b’ means the GST monomer, respectively. d The overall structure of H. parallela GST monomer. GSH binding sites are colored green. e Phylogenetic analysis of H. parallela GST. The red and green circles highlight H. parallela GST and the closely homologous specie GST, respectively. Scale bar, 0.1 substitutions per site.