Table 3 Primers for qRT-PCR validation of candidate miRNAs and mRNAs
| Â | Gene | Primer |
|---|---|---|
miRNA | mmu-miR-141-3p | CGCTAACACTGTCTGGTAAAGATGG |
| Â | mmu-miR-200b-5p | CATCTTACTGGGCAGCATTGGA |
| Â | mmu-miR-34a-5p | TGGCAGTGTCTTAGCTGGTTG |
| Â | mmu-miR-380-5p | CGATGGTTGACCATAGAACATGCG |
| Â | mmu-miR-409-3p | CCGAATGTTGCTCGGTGAACC |
| Â | mmu-miR-455-3p | GCAGTCCACGGGCATATACAC |
| Â | mmu-miR-487b-3p | CAATCGTACAGGGTCATCCACTT |
| Â | mmu-miR-683 | CCTGCTGTAAGCTGTGTCCTC |
| Â | mmu-miR-802-5p | GGCCTCAGTAACAAAGATTCATCCTT |
| Â | mmu-miR-96-5p | GTTTGGCACTAGCACATTTTTGCT |
| Â | mmu-miR-99a-5p | CAACCCGTAGATCCGATCTTGTG |
| Â | mmu-let7e-5p | CGCTGAGGTAGGAGGTTGTATAGT |
mRNA | Atp6v0a1 | F: CCGAGGACGAAGTGTTTGACT R: ATCAGCAGGATAGCCACGGT |
| Â | Elovl2 | F: CCTGCTCTCGATATGGCTGG R: AAGAAGTGTGATTGCGAGGTTAT |
| Â | Lamp2 | F: TGTATTTGGCTAATGGCTCAGC R: TATGGGCACAAGGAAGTTGTC |
| Â | Wdr18 | F: TGGTGTGGGAGCTTCATTCG R: CCCAGGCGCAGATGTAGTTC |
| Â | Hmbs | F: ATGAGGGTGATTCGAGTGGG R: TTGTCTCCCGTGGTGGACATA |