Fig. 1

Time course transcriptome analysis evaluating the effect of mitochondrial introgression on the transcriptional response to rapamycin during refeeding. a Male flies were fasted for 12 h followed by treatment for 30 min with 200uM rapamycin or ethanol control on agar followed by refeeding with regular lab food containing 200uM rapamycin or ethanol. Samples were collected for transcriptome analysis at 4 time points including 0 (12 h fasting), 1 (30 min agar + treatment followed by 30 min food + treatment), 2 and 4 h post starvation. b Western blot analysis of total phosphorylated-P70S6K1 for OreR;OreR (red) and sm21;OreR (blue) flies in response to fasting (left), refeeding with control diet (center) or refeeding with food containing 200uM Rapamycin (right). The analysis was performed on whole fly samples in triplicate and the levels were normalized to total actin. Significant differences between the levels found in treated samples and fasted samples were determined using an unpaired t-test p-value cutoff of 0.05 (* = p < 0.05). c Total genes detected by ImpulseDE2 that show a significant response pattern to refeeding over the full 4 h time course within each GxT condition. Genotype by treatment time course conditions from left to right: OreR;OreR control (left blue); OreR;OreR rapamycin (right blue); sm21;OreR control (left red); sm21;OreR rapamycin (right red). d Total genes detected by ImpulseDE2 that show a significantly different response pattern to refeeding with and without rapamycin treatment over the full 4 h time course within a mitonuclear genotype. Left/blue: The total number of genes with a significant difference between the OreR;OreR control and OreR;OreR rapamycin treated time courses. Right/red: The total number of genes with a significant difference between the sm21;OreR control and sm21;OreR rapamycin treated time courses. e Total genes detected by ImpulseDE2 that show a significantly different response pattern between mitonuclear genotypes over the full 4 h time course within control or rapamycin treated conditions. Right/red: The total number of genes with a significant difference between the OreR;OreR rapamycin treated and sm21;OreR rapamycin treated time courses. For all data, a Benjamini-Hochberg FDR adjusted p-value (adj. p-value < 0.05) was used for determining significant differential gene expression