Fig. 2
From: A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeast
Linear amplification by in vitro transcription improves precision of barcode abundance measurements. a, b Schematics of barcode library generation by a in vitro transcription followed by RT-PCR and b direct PCR amplification. c, d Barcode read counts in libraries prepared from replicate DNA samples by c IVT-RT-PCR and d direct PCR. e Dispersion between replicate measurements as a function of read count