Fig. 4

Pooled competitive growth of a diverse guide RNA library. a Schematic of the competitive growth experiment. b Cells expressing the dCas9-Mxi effector protein are transformed with guide RNA expression plasmids and selected under non-inducing conditions. c Upon guide induction, dCas9-Mxi binds target gene promoters and reduces transcription. d, e Replicate competitive growth experiments. Dilution rate corresponds to growth rate after cultures reach the target cell density. Timepoints for guide RNA induction and initial sampling is shown, along with subsequent sampling timepoints