Comparative transcriptome analysis reveals ectopic delta-5 and delta-6 desaturases enhance protective gene expression upon Vibrio vulnificus challenge in Tilapia (Oreochromis niloticus)

Table 1 Details of RNA sequence read

Sample	Clean	Filtered	Mapping rate
Sample	Reads	Reads	(%)
WT-Liver-ctrl	50,952,012	48,315,226	92.12
WT-Liver-6 h	40,765,142	38,578,158	89.24
WT-Liver-24 h	36,947,506	35,079,100	92.19
D56-Liver-ctrl	39,667,224	37,449,898	90.62
D56-Liver-6 h	52,706,028	49,652,212	88.48
D56-Liver-24 h	54,266,436	50,987,302	91.06

RNA depletion of rRNA and organelle RNA was extracted from liver samples of wild-type and transgenic Tilapia fish expressing delta-6 desaturase plus delta-5 desaturase (D56) at 0, 6 and 24 h V. vulnificaus post infected conditions. The RNA was subjected to multiplexed RNA sequence. The total number of clean reads, filtered reads and RNA mapped reads for the six groups were shown in the table

ISSN: 1471-2164