Identification and evolution analysis of the JAZ gene family in maize

BMC Genomics

Table 2 Results of distances and codon-based Z tests for purifying selection between maize and other plant species for orthologs JAZ groups

Ortholog	maize-rice		maize-sorghum		maize-brachypodium		maize-Arabidopsis		dS-dN Stat from test of
clade	dS	dN	dS	dN	dS	dN	dS	dN	dS > dN (purifying selection)
JAZ1	0.426 ± 0.042	0.202 ± 0.025	0.143 ± 0.029	0.074 ± 0.014	0.410 ± 0.042	0.231 ± 0.026	0.680 ± 0.039	0.507 ± 0.031	6.117*
JAZ2	0.316 ± 0.044	0.149 ± 0.025	0.129 ± 0.034	0.043 ± 0.014	0.325 ± 0.046	0.126 ± 0.024	0.654 ± 0.047	0.462 ± 0.038	5.250*
JAZ3	0.410 ± 0.041	0.162 ± 0.023	0.285 ± 0.034	0.131 ± 0.019	0.391 ± 0.040	0.189 ± 0.026	0.660 ± 0.041	0.499 ± 0.033	7.947*
JAZ4	0.324 ± 0.058	0.281 ± 0.044	0.245 ± 0.050	0.215 ± 0.038	0.340 ± 0.058	0.271 ± 0.045	n/a	n/a	1.532
JAZ5	0.497 ± 0.038	0.222 ± 0.022	0.379 ± 0.035	0.171 ± 0.018	0.478 ± 0.038	0.234 ± 0.024	0.683 ± 0.034	0.593 ± 0.030	8.495*
JAZ6	n/a	n/a	n/a	n/a	n/a	n/a	n/a	n/a	4.287*
Overall	0.522 ± 0.064	0.308 ± 0.048	0.516 ± 0.063	0.274 ± 0.045	0.533 ± 0.064	0.305 ± 0.047	0.704 ± 0.068	0.364 ± 0.051	7.402*

*Estimations of synonymous and nonsynonymous distance between two species are referred as dS and dN, respectively. To be considered under purify selection, a dN/dS ratio less than 1 (dS > dN) and a p-value for the Z-test below 0.05 were required (*, P < 0.05). According to these criteria, almost all JAZ genes were determined to be under purify selection, except for JAZ group 4 which was under neutral selection. Sixty JAZ sequences in total were included in this analysis

ISSN: 1471-2164