Fig. 5

Fine mapping of variants associated with male fertility on BTA26. a Zoom on the Manhattan plot on chromosome 26, 49.5 to 51.5 Mb, of the whole-genome GWAS on bull fertility with recessive model. Bonferroni corrected significant variants are displayed in red. The ENSBTAG00000019919 missense variant (BTA26:50,850,915C > G) is indicated with a black dot. Symbols over the Manhattan plot indicate genes and transcripts that are annotated at the QTL region. Blue and violet colour indicates genes and transcripts with testis-biased expression and male and female reproductive tract specific expression. b Expression of genes between 49.5 and 51.5 Mb in adult (blue) and newborn (black) bull testis in TPM. Blue and violet labels indicate genes with testis-biased expression and male and female reproductive tract specific expression. c Clustal Omega multi-species alignment of the protein produced from ENSBTAG00000019919 and its homologous genes in Mus musculus (ENSMUST00000129990.8), Sus scrofa (ENSSSCT00000011782.4), Equus caballus (ENSECAT00000077103.1), Homo sapiens (ENST00000368586.10), Canis lupus familiaris (ENSCAFT00000017743.4), Ovis aries (ENSOART00000018738.1), Bos mutus (ENSBMUT00000020335.1), Bos taurus wildtype (ENSBTAT00000026536.5), and Bos taurus mutant (N576K)