Fig. 4
From: Transcriptional profiling of identified neurons in leech
The ip3rb2 transcript localizes to T neurons. Left panel, brightfield micrograph: ISH for ip3rb2 shows three bilateral pairs of neurons as expected for the T cells. Center panel, fluorescence micrograph: prior to the in situ staining, two of the three T neurons on the left hand side (white arrowheads) had been injected with RDA (red). Right panel, fluorescence micrograph: magnified view of the boxed region in center panel shows that RDA signal in neuronal somata is masked by ISH product. Background signal is due to autofluorescence arising during ISH processing