Table 3 Genes Used for in situ Hybridization Verification of RNASeq Libraries
From: Transcriptional profiling of identified neurons in leech
Common Name | Cluster | Trinity Gene ID | Helro BLAST (evalue) | SWISS Prot BLAST (evalue) | Average Expression in Each Cell Type in TPM (Transcripts per Kilobase Million) +/− SEM | ||||
|---|---|---|---|---|---|---|---|---|---|
T | P | N | Gm | R | |||||
Aromatic Amino Acid Decarboxylase | 2 | comp24417_c0 | jgi|Helro1|186120 (6.91E-70) | P14173.1 (3.12E-6) | 13.7 +/− 2.7 | 8.2 +/−  2.3 | 17.7 +/−  3.1 | 12.5 +/−  2.4 | 2432.5 +/−  465.0 |
Tryptophan Hydroxylase | 2 | comp20323_c0 | jgi|Helro1|79745 (0) | P70080.1 (1.13E-15) | 5.7 +/− 1.0 | 5.5 +/−  1.0 | 5.2 +/− 2.2 | 32.5 +/−  3.1 | 929.0 +/−  28.0 |
HCN Channel | 4 | comp20462_c0 | jgi|Helro1|98055 (8.90E-126) | O88703.1 (10E-101) | 2.8 +/− 1.0 | 31.6 +/− 12.9 | 7.4 +/− 2.0 | 1.3 +/−  0.3 | 0.5 +/−  0.2 |
Voltage-Gated Potassium Channel | 4 | comp25036_c1 | jgi|Helro1|64112 (4.92E-83) | Q9H252 (3.26E-52) | 6.9 +/− 2.0 | 26.1 +/− 11.0 | 5.3 +/− 3.8 | 1.22 +/−  0.3 | 0.0 +/−  0.0 |
Protocadherin | 4 | comp15454_c0 | jgi|Helro1|69346 (1.50E-77) | Q9BZA7.1 (2.58E-48) | 32.6 +/− 13.8 | 511.8 +/−  68.4 | 235.6 +/−  92.2 | 14.3 +/−  1.8 | 7.8 +/−  5.6 |
Collagen-alpha | 3 | comp13598_c0 | jgi|Helro1|110155 (2.52E-17) | Q17RW2.2 (3.39E-11) | 23.5 +/− 5.7 | 0.2 +/ 0.1 | 5.2 +/− 1.0 | 0.6 +/ 0.3 | 0.04 +/− 0.04 |
Inositol Triphosphate Receptor | 3 | comp24045_c0 | jgi|Helro1|162846 (1.84E-69) | P70227.3. (1.44E-06) | 521.5 +/− 186.7 | 22.9 +/− 5.6 | 105.8 +/− 17.4 | 6.9 +/−  1.3 | 3.0 +/ 2.7 |
Annelid Hypothetical | 5 | comp21991_c0 | jgi|Helro1|169916 (6.50E-29) | None | 42.1 +/− 14.8 | 179.6 +/− 80.7 | 103.5 +/− 10.0 | 7.3 +/− 0.7 | 1.0 +/−  0.4 |