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Table 3 Genes Used for in situ Hybridization Verification of RNASeq Libraries

From: Transcriptional profiling of identified neurons in leech

Common Name Cluster Trinity Gene ID Helro BLAST (evalue) SWISS Prot BLAST (evalue) Average Expression in Each Cell Type in TPM (Transcripts per Kilobase Million) +/− SEM
T P N Gm R
Aromatic Amino Acid
Decarboxylase
2 comp24417_c0 jgi|Helro1|186120 (6.91E-70) P14173.1 (3.12E-6) 13.7 +/− 2.7 8.2 +/−  2.3 17.7 +/−  3.1 12.5 +/−  2.4 2432.5 +/−  465.0
Tryptophan Hydroxylase 2 comp20323_c0 jgi|Helro1|79745 (0) P70080.1 (1.13E-15) 5.7 +/− 1.0 5.5 +/−  1.0 5.2 +/− 2.2 32.5 +/−  3.1 929.0 +/−  28.0
HCN Channel 4 comp20462_c0 jgi|Helro1|98055 (8.90E-126) O88703.1 (10E-101) 2.8 +/− 1.0 31.6 +/− 12.9 7.4 +/− 2.0 1.3 +/−  0.3 0.5 +/−  0.2
Voltage-Gated Potassium Channel 4 comp25036_c1 jgi|Helro1|64112
(4.92E-83)
Q9H252
(3.26E-52)
6.9 +/− 2.0 26.1 +/− 11.0 5.3 +/− 3.8 1.22 +/−  0.3 0.0 +/−  0.0
Protocadherin 4 comp15454_c0 jgi|Helro1|69346 (1.50E-77) Q9BZA7.1 (2.58E-48) 32.6 +/− 13.8 511.8 +/−  68.4 235.6 +/−  92.2 14.3 +/−  1.8 7.8 +/−  5.6
Collagen-alpha 3 comp13598_c0 jgi|Helro1|110155 (2.52E-17) Q17RW2.2 (3.39E-11) 23.5 +/− 5.7 0.2 +/ 0.1 5.2 +/− 1.0 0.6 +/ 0.3 0.04 +/− 0.04
Inositol Triphosphate Receptor 3 comp24045_c0 jgi|Helro1|162846 (1.84E-69) P70227.3. (1.44E-06) 521.5 +/− 186.7 22.9 +/− 5.6 105.8 +/− 17.4 6.9 +/−  1.3 3.0 +/ 2.7
Annelid Hypothetical 5 comp21991_c0 jgi|Helro1|169916 (6.50E-29) None 42.1 +/− 14.8 179.6 +/− 80.7 103.5 +/− 10.0 7.3 +/− 0.7 1.0 +/−  0.4