Fig. 2

RNAseq comparison of MVC_eGFP vs. OSN_eGFP- cells. a Heatmaps showing hierarchical clustering of the top 10 upregulated and top 10 downregulated genes identified by DESeq2. b Heatmaps showing hierarchical clustering of the 550 olfactory receptor genes identified by DESeq2 as expressed in OSN_eGFP- cells. For both a and b, row and column order were determined automatically by the pHeatmap package in R. Row values were centered and scaled using ‘scale = “row”’ within pHeatmap. c Volcano plot of all olfactory receptors, demonstrating the large number of enriched olfactory receptors in the OSN_eGFP- population. d Hierarchical clustering of transcripts for taste transduction and transcripts expressed in canonical and non-canonical OSNs identified by RNAseq as significantly different in expression between MVC_eGFP and OSN_eGFP- cells. The non-canonical OSNs considered here included guanilyl-cyclase D (GC-D) OSNs [37], Trpc2 OSNs [64], Cav2.1 OSNs [72], and OSNs expressing trace amine-associated receptors (Taars) [46]. Transcripts identified by DESeq2. e Gene ontology (GO) term enrichment for synaptic vesicle or chemosensory-related GOs was calculated from differentially expressed genes using TopGO in R. An enrichment value for genes with Fischer p value < 0.05 was calculated by dividing the number of expressed genes within the GO term by the number expected genes (by random sampling, determined by TopGO)