Fig. 5

In situ hybridization chain reaction finds strong TRPM5 mRNA expression in MVC_eGFP cells, but not in the nuclear OSN layer. a In situ for TRPM5 (yellow) and OMP (magenta) transcripts in the olfactory epithelium of TRPM5-GFP mice (GFP is green) shows strong label for TRPM5 in MVCs (asterisks) and sparse labeling in the OSN nuclear layer (arrows). The scale bar is 20 μm. b In situ for TRPM5 (yellow) and OMP (magenta) transcripts in the olfactory epithelium of TRPM5-GFP x TRPM5-knockout mice (GFP is green) shows no label for TRPM5 in GFP-positive MVCs (asterisks) and does show sparse labeling in the OSN nuclear layer (arrows). The scale bar is 20 μm