Table 1 List of key resources and reagents used in this study
REAGENT TYPE | REAGENT or RESOURCE | SOURCE | IDENTIFIER | ADDITIONAL INFORMATION |
|---|---|---|---|---|
Chemical compound, drug | BrainPhys Neuronal Medium | Stemcell Technologies | Â | Product # 05791 |
Chemical compound, drug | Dispase II | Sigma | Â | Product # D4693 |
Chemical compound, drug | AcGFP1/eGFP calibration beads | Takara | Â | Flow cytometry calibration beads Product # 632594 |
Chemical compound, drug | mCherry calibration beads | Takara | Â | Flow cytometry calibration beads Product # 632595 |
Chemical compound, drug | RQ1 RNase-free DNase | Promega | Â | Product # M6101 |
Chemical compound, drug | Papain | Sigma | Â | Product # P3125 |
Chemical compound, drug | Paraformaldehyde (32%) | Electron Microscopy Sciences | Â | Product # 157145 |
Chemical compound, drug | RNAprotect Tissue Reagent | Qiagen | Â | Product # 76526 |
Chemical compound, drug | RNeasy Plus Micro Kit | Qiagen | Â | Product # 74034 |
Chemical compound, drug | High Capacity c-DNA Reverse Transcription kit | ABI | Â | Â |
Chemical compound, drug | 18 s rRNA | PE ABI |  |  |
Strain, strain background | TRPM5-eGFP | Dr. Robert Margolskee [14] | Â | Â |
Strain, strain background | TRPM5 knockout | Dr. Robert Margolskee [16] | Â | Â |
Strain, strain background | OMP-H2B::Cherry | Generated for this publication | Â | This mouse will be deposited in Jackson Laboratories |
Software, algorithm | MATLAB_R2018a | Mathworks | RRID: SCR_001622 | Â |
Software, algorithm | Illustrator | Adobe | RRID: SCR_010279 | Â |
Software, algorithm | Photoshop | Adobe | RRID: SCR_014199 | Â |
Software, algorithm | InDesign | Adobe | Â | Â |
Software, algorithm | MoFlo Astrios Summit Software (6.3.1.16945). | Beckman Coulter | Â | Â |
Software, algorithm | BBMap (BBDuk) | Â | RRID:SCR_016968 | Â |
Software, algorithm | Salmon v1.2.1 | RRID:SCR_017036 | [67] | |
Software, algorithm | DeSEQ2 v1.28.0 | https://bioconductor.org/packages/release/bioc/html/DESeq2.html | RRID:SCR_015687 | [51] |
Software, algorithm | TopGO, v2.40.0 | Â | RRID:SCR_014798 | Â |
Software, algorithm | pHeatmap, 1.0.12 | Â | RRID:SCR_016418 | Â |
Software, algorithm | Ensembl GRCm38, v99 | Â | Â | Â |
Software, algorithm | R, v4.0 | Â | RRID:SCR_001905 | Â |
Software, algorithm | Tximport, v1.16.0 | Â | RRID:SCR_016752 | Â |
Software, algorithm | SAMtools | SAMtools | RRID:SCR_002105 | [44] |
Software, algorithm | Bedtools | Â | RRID:SCR_006646 | Â |
Software, algorithm | STAR v2.5.3a | RRID:SCR_015899 | Â | |
Software, algorithm | Sigmaplot, v12.5 | Systat Software | RRID:SCR_003210 | Â |
Software, algorithm | Custom code for bioinformatics analysis | Â | Â | |
Software, algorithm | OPM | Â | Â |