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Table 5 Metabolite concentrations (ng/mg of total protein) in liver tissue from 4-d old Holstein calves (n = 6/group) born to cows randomly assigned to receive a basal control (CON) diet from −28 ± 2 d to parturition [1.47 Mcal/kg dry matter (DM) and 15.3% crude protein (CP)] with no added Met or CON plus ethyl cellulose Met (MET, Mepron®, Evonik Nutrition & Care GmbH, Germany)

From: Unique adaptations in neonatal hepatic transcriptome, nutrient signaling, and one-carbon metabolism in response to feeding ethyl cellulose rumen-protected methionine during late-gestation in Holstein cows

Metabolite, (ng/mg of total protein) Maternal diet SEM p-value
CON MET
1-Carbon metabolism
 Adenosine 3,855 8,411 1,052 0.02
 Methionine 979.29 1,566 254.08 0.29
 Betaine 15,282 21,058 3,424 0.44
 Choline 3,165 4,550 500.53 0.19
 Glycine 9,643 19,085 2,035 0.01
 5-Methyltetrahydrofolic acid 8.06 12.08 2.67 0.49
 N,N-Dimethylglycine 33.67 55.76 7.74 0.18
 S 5′ Adenosyl homocysteine 320.39 426.08 55.01 0.37
 S 5′ Adenosyl methionine 773.97 630.38 74.81 0.36
 Butyrobetaine 69.95 112.76 14.61 0.17
Transsulfuration
 Cystathionine 346.41 738.41 95.37 0.04
 Cysteine 1,438 2,898 437.86 0.12
 Cysteinesulfinic acid 641.98 1,471 213.39 0.06
 Glutamylcysteine 909.72 1,041 144.79 0.67
 Glutathione 19,575 21,762 2,557 0.69
 Hypotaurine 2,929 5,879 1,080 0.20
 Serine 3,663 7,387 770.19 0.01
 Taurine 56,308 117,719 13,488 0.03
Tricarboxylic acid cycle
 Ketoglutaric acid 468.75 541.95 57.50 0.55
 Fumarate 1,775 3,504 419.50 0.04
 Glutamate 33,518 65,748 7,593 0.04
 NAD 10,074 23,436 2,829 0.01
 FAD 2,563 4,017 464.71 0.14
 NADH 3,796 7,733 994.65 0.05
 Pyruvate 1,656 1,974 293.37 0.62
 Malic acid 7,591 8,053 1,367 0.90
 NAD/NADH 2.75 3.68 0.47 0.35
Other
 Glutamine 2,567 5,256 1,216 0.36
 Carnitine 688.15 1170 160.62 0.16
Bile acids
 Glycocholic acid 6,444 10,845 1,054 0.05
 Glycochenodeoxycholic acid 2,627 5,438 666.36 0.04
 Taurocholic acid 1,829 3,106 271.17 0.02
 Lithocholic acid 7,796 26,284 3,501 0.01
 Taurochenodeoxycholic acid 1,738 3,010 320.82 0.06