Fig. 5

Variation on gene expression of S. ixodetis DCF in the gut, ovary, and hemolymph of D. coccus. (a) Heatmap showing all the differentially expressed genes in each tissue comparison (i.e. gut vs hemolymph, gut vs ovary and hemolymph vs ovary). Color range in heatmaps indicates variation in log2 fold-change. Gray boxes indicate no differential expression observed after DESeq2 analysis (absolute log₂ fold-change ≥0.58 and p-adjust value ≤0.05). hyp means genes annotated as hypothetical proteins. (b) Volcano plot of differential gene expression of S. ixodetis DCF in gut vs hemolymph, gut vs ovary and hemolymph vs ovary comparisons. Red dots show genes considered as differentially expressed after DESeq2 analysis absolute log₂fold change > 0.58 (outer broken vertical lines) and p-adjust value ≤0.05 (−log₁₀ p-value ≥1.3, broken horizontal line). Green dots show non-differentially expressed genes with an absolute log₂ fold-change > 0.58 and p-adjust value > 0.05. Gray dots show non-differentially expressed genes with an absolute log₂fold change < 0.58 and p-adjust value > 0.05 (non-differentially expressed). All annotated genes are represented, red dots without annotation legend represent hypothetical protein coding genes differentially expressed