Fig. 3
From: Methamphetamine-induced changes in myocardial gene transcription are sex-dependent
Methamphetamine selectively regulates transcription of circadian clock-related genes in female hearts. Methamphetamine-induced changes in genes that regulate the circadian rhythm were identified by RNA sequencing and qPCR. RNA sequencing identified significant effects of methamphetamine on the number of transcripts encoding Per2 a, Per3 c, Dbp e, Bmal1 g, Npas2 i, Clock k, and Cry2 m. Two-way ANOVA of qPCR data indicated significant effects of methamphetamine on the number of transcripts encoding Per2 [F = 17 [1, 20], p < 0.0005] b, Dbp [F = 47 [1, 20], p < 0.0001] f, BmalI [F = 51 [1, 20], p < 0.05] h, and Npas2 [F = 19 [1, 19], p < 0.0005] j. There was also a significant interaction between sex and methamphetamine on Npas transcription [F = 8 [1, 19], p < 0.01] j. a indicates padj < 0.0001 compared to hearts from saline treated females. b indicates padj < 0.00005 compared to hearts from saline treated females. c indicates padj < 0.0005 compared to hearts from saline treated females. d indicates padj < 0.001 compared to hearts from saline treated females. e indicates p < 0.05 vs female saline; f indicates p < 0.0005 vs female saline; g indicates p < 0.0005 vs male saline. Data represent the mean ± SEM of 5–6 animals