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Fig. 5 | BMC Genomics

Fig. 5

From: Strategies for detecting and identifying biological signals amidst the variation commonly found in RNA sequencing data

Fig. 5

Trendline gene expression profiles remain consistent over a wide range of RNA-seq expression levels. Four genes displaying unadjusted gene counts ranging from 9 to 3836 counts were rank-ordered (left panel). Gene trendline linearity was independent of the level of gene expression. ERCC spike-in standards also showed linearity but the ERCC standard in index 35 (sample 7) was markedly elevated (right panel). However, sample 7 did not display similar deviations in any of the four genes depicted in the left panel of Fig. 5, which suggest a spike-in issue with little or no technical impact on gene counts. Red circles identify sample 7

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BMC Genomics

ISSN: 1471-2164

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