Table 1 Statistics of raw reads and assembled transcripts from Roche 454 and Illumina reads
Basic statistics of raw reads | |
Total number of obtained raw reads | 150,022,805 |
Illumina MiSeq | 149,697,864 |
Roche 454 | 324,941 |
Average length of obtained raw reads | |
Illumina MiSeq | 100 bp |
Roche 454 | 424 ± 219 bp |
Total number of processed reads before assembly | 123,774,558 |
Illumina MiSeq | 123,555,644 |
Roche 454 | 218,914 |
Statistics of transcriptome assembly | |
Total number of final transcripts | 37,062 |
Mean length of nucleotide sequences | 736 bp |
Number of transcripts encoding full-length protein ≥30 amino acids | 14,203 (38.32%) |
Number of transcripts with start codon (ATG) only | 3689 (9.95%) |
Number of transcripts terminated by a stop codon only | 13,092 (35.32%) |
Complete/partial matches to 248 CEGMA core proteins | 69.35, 90.73% |
978 searched BUSCO groups (%) | Comp: 776 (79.34) [Single 436 (44.58), Dupl: 340 (34.76)], Fragm: 63 (6.44), Missing: 139 (14.21) |
GC content in transcripts | 42.30% |
N50 | 1548 bp |
N90 | 360 bp |