Fig. 3

Quantitative FISH validates consensus centromere satellite copy number variation across inbred mouse strains. a Representative FISH images from four genetically diverse inbred strains: CAST/EiJ, WSB/EiJ, LEWES/EiJ, and PWK/PhJ. Individual color channels were manually manipulated using the Color Balance feature in FIJI for presentation purposes. Only raw, unedited images were used for quantification. Quantification of fluorescent intensity using DNA probes derived from the (b) major and (c) minor centromere satellite repeats across inbred strains. Points correspond to fluorescent intensity measurements for a single chromosome. A minimum of 40 centromeres from 36 cells were examined per strain. Fluorescent intensity is represented in arbitrary units (AU)