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Fig. 8 | BMC Genomics

Fig. 8

From: Comprehensive analysis and identification of drought-responsive candidate NAC genes in three semi-arid tropics (SAT) legume crops

Fig. 8

Validation of expression profiles of selected NAC genes in contrasting drought-responsive genotypes of the three legume crops. a Expression of selected Ca_NAC genes in chickpea root tissues under drought stress treatment. Expression data were obtained by qRT-PCR of drought-stressed and well-watered root samples of 30-day-old chickpea plants. Root tissues were collected after six days of drought induction. Mean relative expression levels were normalized to a value of 1 in control root samples. Fourteen of fifteen selected genes (except Ca_05227) were examined. Error bars = SE values of two biological replicates and three technical replicates. Significant differences were determined by Student’s t-test at P ≤ 0.05. b Expression of selected Cc_NAC genes in pigeonpea root tissues under drought stress treatment. Expression data were obtained by qRT-PCR of drought-stressed and well-watered root samples of 30-day-old pigeonpea plants. Root tissues were collected after six days of drought induction. Mean relative expression levels were normalized to a value of 1 in control root samples. Error bars = SE values of two biological replicates and three technical replicates. Significant differences were determined by Student’s t-test at P ≤ 0.05. (c) Expression of selected Ah_NAC genes in groundnut root tissues under drought stress treatment. Expression data were obtained by qRT-PCR of drought-stressed and well-watered root samples of 30-day-old groundnut plants. Root tissues were collected after six days of drought induction. Fourteen selected genes were examined. Mean relative expression levels were normalized to a value of 1 in control root samples. Error bars = SE values of two biological replicates and three technical replicates. Significant differences were determined by Student’s t-test at P ≤ 0.05

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