Table 2 Summary of genome statistics for High-quality MAGs compared to representatives on the public datasets. Coverage (Cov.) and circularity (Circ.) retrieved from Flye; completeness (% Compl.), from CheckM; tRNAs and rRNA values, from PROKKA. tRNAs count refers to unique canonical tRNAs. GTDB species representative are used as the references for comparison. The two exceptions are Succinivibrio and Sutterella since they were potential novel species regarding GTDB, but we found a MAG > 95% ANI on the animal gut metagenome and UHGG catalog, respectively. apartial gene call by PROKKA. bnot detected by PROKKA, but the GTDB/NCBI reference for Blautia argii (GCF_003287895.1) is described to contain five 5S rRNA genes
From: Long-read metagenomics retrieves complete single-contig bacterial genomes from canine feces
| Â | Length (Mbp) | Cov. | Circ. | % Compl. | CDS | tRNAs | 16S rRNA | 23S rRNA | 5S rRNA | % pseudo genes | Contiguity level |
|---|---|---|---|---|---|---|---|---|---|---|---|
Succinivibrio sp. | 2.04 | 101X | N | 98.68 | 1683 | 20 | 7 | 7 | 8 | 0.83% | 1 contig |
Animal Gut Mg: Freddie_038 MAG | 1.74 | – | N | 97.50 | 1490 | 14 | 0 | 0 | 0 | 0.20% | 185 contigs |
Sutterella sp. | 2.70 | 123X | N | 95.49 | 2646 | 19 | 9 | 9 | 0 | 3.86% | 1 contig |
UHHG: MGYG_HGUT_01574 MAG | 1.14 | – | N | 78.72 | 1012 | 14 | 0 | 0 | 0 | 0.69% | 24 contigs |
Prevotellamassilia sp900541335 | 2.72 | 577X | Y | 97.65 | 2037 | 20 | 7 | 7 | 7 | 0.15% | 1 contig |
GTDB Rep: GCA_900541335.1 | 2.42 | 13X | N | 96.13 | 1842 | 16 | 0 | 0 | 0 | 0.11% | 95 contigs |
Phascolarctobacterium sp900544885 | 2.09 | 205X | N | 99.85 | 1985 | 20 | 5 | 5 | 5 | 1.61% | 1 contig |
GTDB Rep: GCA_900544885.1 | 1.75 | 20X | N | 98.65 | 1610 | 18 | 1a | 0 | 0 | 0.68% | 87 contigs |
Catenibacterium sp000437715 | 2.53 | 17X | N | 98.50 | 2565 | 20 | 7 | 7 | 7a | 1.83% | 1 contig |
GTDB Rep: GCF_004168205.1 | 2.54 | 45X | N | 100 | 2003 | 20 | 1a | 0 | 0 | 0.90% | 212 contigs |
Blautia sp900541345 | 2.44 | 44X | N | 93.86 | 2313 | 19 | 6 | 6 | 6 | 1.30% | 1 contig |
GTDB Rep: GCA_900541345.1 | 2.69 | 8X | N | 95.85 | 2499 | 16 | 0 | 0 | 0 | 0.72% | 160 contigs |
Enterococcus_B hirae | 2.78 | 42X | Y | 99.13 | 2511 | 20 | 6 | 6 | 6 | 1.27% | 1 contig |
GTDB Rep: GCF_000271405.2 | 2.83 | – | Y | 99.63 | 2670 | 20 | 6 | 6 | 6 | 0.97% | Complete |
Blautia sp003287895 (Blautia argi) | 2.96 | 31X | N | 92.78 | 2698 | 20 | 5 | 5 | 0b | 0.59% | 1 contig |
GTDB Rep: GCF_003287895.1 | 3.30 | 217X | Y | 97.64 | 3203 | 20 | 5 | 5 | 0b | 2.06% | Complete |