Fig. 3

Power calculations using MAST with a random effect for individual. Power curves for various, but likely, single-cell scenarios using MAST with a random effect for individual. Power is computed at α = 0.05. Panel a demonstrates differences in power when sample sizes range between 5 individuals per group to 100 when the number of cells per individual is held constant at 250. Panel b demonstrates the differences in power when increasing the number of cells per individual (100, 250, 500, 1000) for 10 individuals per group. Panels c and d demonstrate the very minor differences in power by increasing the number of cells per individual (100, 250, 500, 1000) when testing for association with a continuous trait for 20 individuals and 100 individuals, respectively