Table 1 The seven treatment sub-groups for sperm-surface protein extraction from two treatment classes and the corresponding TPP results indicating total spectra, correctly and incorrectly identified proteins at p ≥ 0.6 and 0.99
Sample | PEP-XML Total spectra | Ipro.pep.xml Total spectra | Prot.xml Total proteins | Total proteins with p ≥ 0.6 | Incorrectly identified with iProphet p ≥ 0.6 | Total proteins with p ≥ 0.99 | Incorrectly identified with iProphet p ≥ 0.99 |
|---|---|---|---|---|---|---|---|
2x-DPBS-30 min | 26,535 | 7252 | 1733 | 875 | 76 | 317 | 0 |
4x-DPBS-30 min | 26,470 | 8399 | 1725 | 956 | 76 | 391 | 0 |
2x-DPBS-60 min | 25,947 | 7742 | 1898 | 1013 | 90 | 395 | 0 |
4x-DPBS-60 min | 25,951 | 8210 | 2162 | 1244 | 99 | 432 | 0 |
1 U/ml | 24,829 | 6557 | 1788 | 992 | 99 | 385 | 0 |
1.5 U/ml | 24,587 | 6343 | 1932 | 1005 | 76 | 353 | 0 |
2 U/ml | 24,881 | 5978 | 1662 | 793 | 63 | 364 | 0 |