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Fig. 3 | BMC Genomics

Fig. 3

From: Comprehensive analysis of differentially expressed circRNAs and ceRNA regulatory network in porcine skeletal muscle

Fig. 3

Experimental validation of DEcircRNAs. a Divergent and convergent primers amplify circRNA results in cDNA and gDNA samples. b Sanger sequencing confirmed the back-splicing junction sequence of circRNAs (Blue arrow points to the splicing site). c qRT-PCR results showing the resistance of circRNAs and linear genes to RNase R digestion. Note: Elongation factor Tu GTP binding domain containing 2 (EFTUD2), La ribonucleoprotein 4B (LARP4B), Reticulon 4 (RTN4) and Senataxin (SETX) were the host genes of circ_0094, circ_009145, circ_0017653, and circ_0014301 respectively; * represents significant difference (P < 0.05), ** represents extremely significant difference (P < 0.01)

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BMC Genomics

ISSN: 1471-2164

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