Fig. 7

Hypothetic roles of C. aegerita orthologs of “classic” model agaric fruiting-related genes (FRGs) from S. commune, C. cinerea and C. aegerita SM51 in FB development of C. aegerita AAE-3. a Landmarks during fruiting of C. aegerita AAE-3 in the fruiting regime of Herzog et al. [6] as monitored by handcuts. The five stages of this process are shown in white to orange boxes. Scale bars represent 100 μm (white box), 400 μm (light yellow box), 0.5 mm (yellow box), 1 mm (dark yellow box) and 2 mm (orange box). b Model for the regulation of C. aegerita AAE-3 FB development. Indices I and II symbolize consecutive fruiting flushes that are typical for C. aegerita fructification. Green lines represent a presumed functional role in a certain stage by the respective putative C. aegerita AAE-3 orthologs of proteins with experimentally ascertained function from S. commune and C. cinerea. These presumptions are necessarily also based on the differential expression data of the corresponding C. aegerita-FRG from the present study. For the sake of simplicity this categorization also extends to the C. aegerita AAE-3 counterparts of the PRI1 to PRI4 genes of C. aegerita SM51 where functions were presumed based on transcription profiling and in silico analyses. Putative interaction between Dst1 and Bwc2 is symbolized by a thin line ending in a diamond on each side. Blue lines represent hypothetical roles within C. aegerita AAE-3 fruiting body development solely based on the expression data recorded within the present study. Dotted lines represent conjectured (in) direct transcriptional activation/repression of target genes based on publications on putative counterparts from another mushroom. Grayed-out genes did not show differential expression in the present study in the respective developmental stage(s) of C. aegerita AAE-3 but have previously been shown to trigger relevant fruiting body developmental processes in the model mushroom S. commune [10, 12]