Fig. 2

Functional miRNA absence in the B⁺ genome assembly. a The novel_2026-B⁺ predicted from the B⁺ assembly. This miRNA has a stem-loop secondary structure. b The NODE_313069 qPCR for B⁻ and B⁺ DNA samples. The difference between B⁻ and B⁺ amplification was not significant (p-value 13 > 0.05). c Predicted novel_2026-B⁺ match with M. zebra scafold_77. The A. latifasciada genomic sequencing reads are shown below the M. zebra reference genome, F1-0B SNP coverage (female B⁻ sample), F-1B SNP coverage (female B⁺ sample), M1-0B SNP coverage (male B⁻ sample), M3-1B SNP coverage (male B⁺ sample). The gray area is the read coverage, the blank spaces show no aligned reads in this region, and the red rectangle highlights the novel_2026-B⁺ NODE_313069 region matching the M. zebra assembly