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Table 1 Quality assessment metrics of unfiltered and filtered transcriptome assemblies for multiple tissue types collected from four marine turtle species

From: Species and population specific gene expression in blood transcriptomes of marine turtles

 

Loggerhead - blood

Hawksbill - blood

Green turtle - blood

Leatherback - blood

Leatherback - brain

Leatherback - lung

Leatherback - ovary

raw

filtered

raw

filtered

raw

filtered

raw

filtered

raw

filtered

raw

filtered

raw

filtered

Total trinity transcripts

132,146

77,392

280,711

220,458

489,355

376,736

347,717

276,709

216,942

140,332

243,118

165,611

163,840

119,574

Contig N50

3032

2552

3143

2276

3221

2303

2867

2187

3618

2788

3288

2526

3050

2373

Median contig length

675

707

574

529

606

575

597

553

666

629

632

601

673

593

Mean mapping rates

 Conspecific samples

91.50%

75.36%

95.53%

93.58%

94.88%

93.94%

95.49%

94.95%

92.98%

83.22%

92.52%

82.02%

94.96%

93.89%

 Heterospecific samples

82.65%

69.54%

88.56%

85.44%

86.24%

85.99%

83.58%

83.14%

N/A

N/A

N/A

N/A

N/A

N/A

Transrate scores

 Assembly score

0.23

0.35

0.25

0.36

0.29

0.42

0.26

0.37

0.21

0.31

0.21

0.29

0.20

0.29

 Optimal assembly score

0.35

0.36

0.36

0.37

0.42

0.43

0.36

0.38

0.33

0.32

0.30

0.30

0.30

0.30