De novo transcriptome analysis and comparative expression profiling of genes associated with the taste-modifying protein neoculin in Curculigo latifolia and Curculigo capitulata fruits

Table 5 Summary of neoculin group transcripts in fruits of two Curculigo species

	Transcript ID	Reference transcript	No. of substitutions (amino acid)	Heterodimerization (no. of Cys)	Lectin activity (no. of MBS^a)	Taste modification	Expression (TPM^b)
*C. latifolia*	L_16562_c0_g1_i1 (NBS)	NBS	0	Yes (4)	No (0)	Yes	High (650)
	L_16562_c0_g1_i2 (NAS)	NAS	1	Yes (4)	No (0)	Yes	High (620)
	L_307_c0_g2_i1	C_9931_c0_g1_i1	1	Unknown (2)	Unknown (3)	Unknown	Very low (1.4)
	L_307_c0_g1_i1	C_9931_c0_g1_i1	1	Unknown (2)	Unknown (3)	Unknown	Very low (0.35)
*C. capitulata*	C_16324_c0_g1_i1	NBS	6	Probably Yes (4)	Probably No (0)	Probably Yes	Low (8.0)
	C_16324_c0_g1_i2	NAS	0	Probably Yes (4)	Probably No (0)	Probably Yes	low (11)
	C_9931_c0_g1_i1	C_9931_c0_g1_i1	–	Unknown (2)	Unknown(3)	Unknown (Fruits have no activity)	Very high (15,000)

The number of amino acid residues difference from the reference sequences, the potential for heterodimerization, lectin activity, taste modification, and expression levels of the transcripts from C. latifolia or C. capitulata fruits are summarized. As the reference sequences, amino acid sequences of NBS, NAS, and C_9931_c0_g1_i1 of C. capitulata were used
^aMBS mannose-binding site
^bTPM transcripts per million

ISSN: 1471-2164