ddRAD sequencing: an emerging technology added to the biosecurity toolbox for tracing the origin of brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae)

Table 1 Summary of the in silico and in vitro tests of RE pairs for ddRADseq

RE pairs	In silico: numbers of Segment^a	In vitro: numbers of SNPs^b
MspI-NlaIII	556,040	969
EcoRI-NlaIII	508,739	311
PetI-NlaIII	481,590	Null
MseI-PstI	303,697	17
EcoRI-MseI	296,739	20,136
EcoRI-MluCI	267,087	Null
MspI-MseI	265,748	27,871
PstI-MluCI	259,942	Null
MspI-MluCI	222,963	16,369
MseI-NlaIII	182,338	3909
NlaIII-MluCI	148,911	2135
EcoRI-MspI	118,867	28,328
MseI-MluCI	86,829	Null
MspI-PstI	86,012	Null
EcoRI-PstI	31,417	Null

Note: ^aPrediction of the DNA segments of 300–500 bp against the BMSB genome scaffold
^bTwo replicates were used for each RE pairs. The number showed the shared SNPs between two replicates. Null indicates not tested

ISSN: 1471-2164