Fig. 10

Overall workflow of the experimental setup and bioinformatic analysis performed in this study. Transcriptomic data obtained from NGS were subjected to statistical analysis and quality control prior to functional annotation. Then, the pre-processed transcriptomes were mapped to the reference genome. Unmapped reads against the reference genome were assembled and clustered to produce uni-transcripts data. The newly generated data; uni-transcript were subjected to differential expression analysis that included steps towards novel gene discovery via BLAST2GO enrichment analysis, prediction of ORF gene and TFBS, as well as gene network analysis. Lastly, the selected novel genes identified were determined to perform quantification validation