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Fig. 1 | BMC Genomics

Fig. 1

From: Evaluating the accuracy of Listeria monocytogenes assemblies from quasimetagenomic samples using long and short reads

Fig. 1

The effective time required to sequence and analyze the quasimetagenomic samples. The blue circles marked as 24H, 28H, 32H, 36H, and 40H denote the five enrichment time points where the quasimetagenomic samples were collected and sequenced with the Illumina MiSeq (short read) and the Oxford Nanopore GridIon (long read). Diamonds represent the 30 batches (B1 to B30) of 4000 GridIon reads, each generated 45 min apart. For our analysis, reads from each batch were merged with previously obtained batches to form cumulative batches (Ci). The time taken to assemble the reads is shown with boxes labeled ‘A’. C18 at 24H marks the earliest time point where a complete Listeria monocytogenes genome was reconstructed (with metaFlye). The green circle corresponds to the time required to culture and sequence a pure colony isolate of Listeria monocytogenes i.e. 144 h. Note: bioinformatic analysis can be performed in “real-time” on the GridIon batches as they are output whereas an Illumina MiSeq sequencing run must finish before the bioinformatics can begin. However, for our analysis we partitioned the reads from each MiSeq run into 30 batches—each composed of an equal number of sequenced bases as the GridIon batches

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