Fig. 5
Phenotypic effect of single SNPs and their combination. a The blocks show the exons of EDNRB2. The arrow indicates the direction of gene transcription. The cones represent significant SNPs associated with the EDNRB2 gene. b The phenotypic effect of every single significant SNP was analyzed on the dorsal and ventral sides. P values and R2 values were calculated using a general linear model (GLM). P values less than 0.05 were considered significant. c Relatively high LD was found among the 4 SNPs. The numbers in the diamond represent R2 values. d The FPKM (fragments per kilobase of transcript per million mapped reads) values of the EDNRB2 gene in duck embryos with different melanin deposition areas were calculated based on our previous RNA-seq data [14]. The expression of EDNRB2 showed a significant increasing trend with increasing melanin area. e Changes in the binding sites of Chr4: 10,180,939 T > C and Chr4: 10,190,671 A > T were demonstrated. The sequence used in the analysis was 10 bp of the left and right flanks of the mutation site. f The genotypes were identified using Chr4: 10,180,939 and Chr4: 10,190,671. E.g. When Chr4: 10,180,939 had the C/C allele and Chr4: 10,190,671 had the A/A allele, it would be shown as CCAA. The P values were calculated by T-test, and values less than 0.05 were considered significant