Fig. 4

A subset of higher read depth DNAm sites are over-represented in RRBS datasets. a A line graph of the mean proportion of DNAm points remaining (y-axis) after filtering by increasing read depth thresholds (x-axis). b The Spearman’s correlation of read depth between all pairs of samples. c The proportion of overlap in the DNAm points present across an increasing number of samples compared. d Read depth plotted from two randomly selected samples, colored by the number of DNAm points that the DNAm site that have a read depth > 0. 1000 DNAm points were randomly selected and read depth is plotted up to 200 to facilitate the interpretation of plots. e The proportion of DNAm sites in intergenic regions (purple), CpG islands (blue), shelves (green) and shores (yellow) for all DNAm sites and all DNAm sites with read depth > 1 across all samples