Fig. 3
From: The holobiont transcriptome of teneral tsetse fly species of varying vector competence
Differentially expressed Wigglesworthia genes between teneral males and females within G. morsitans bacteriome libraries. A A heatmap with row-normalized expression levels are shown where each row represents a gene and each cell represents the relative expression level for a sample in terms of Z-scores [observed transcripts per million (TPM) minus row mean TPM, divided by the standard deviation of TPMs for that row]. Values higher than the row mean are represented in yellow, and values lower than the row mean are represented in blue. Gene names are shown on the right. B Validation of selected Wigglesworthia genes found to be differentially expressed between G. morsitans isolates of females and males. Fold change as estimated by the 2-ΔΔCt method via qRT-PCR supports that these genes are upregulated in females. The bacteriome is within the blue arrowheads and the midgut section is within the pink arrowheads (Created from Biorender.com)