Fig. 8

Transcriptomic profiles of tsetse genes within teneral tsetse flies. A Scatterplot with TPM distribution within each tsetse library; x axis is in log₁₀ scale, only genes with TPM > 0 are plotted (left). The median is indicated with a vertical black line, TPM of 100 is indicated with dotted line; bar graphs partition the percentage of the total number of genes according to their expression levels (low expression < 100 TPM, high expression > 100 TPM or no expression with TPM = 0). The call-outs on the side of each corresponding bar (right) indicate the percentage of genes with > 100 TPM. Black dots at the left and right indicate the median and the cutoff of 100 TPM, respectively. B PCA analysis indicates that 55.4% of the variability across tsetse expression may be accounted for by tsetse tissue (PC1), while 23.8% of the variability may be accounted for by tsetse species (PC2). C Output of IDEAmex which depicts DE genes within the bacteriomes of tsetse species isolates; the intersect at the center of the Venn diagram contains the tsetse genes found to be differentially expressed by the four informatic approaches as identified adjacent to each oval; n = 3246 DE genes. D Output of IDEAmex which depicts DE genes within the midguts of tsetse species isolates; the intersect at the center of the Venn diagram contains the tsetse genes found to be differentially expressed by the four informatic approaches as identified adjacent to each oval; n = 3134 DE genes