Fig. 7
From: The effect of methanol fixation on single-cell RNA sequencing data
Analysis of the fixation effect in 3’ end biased sequencing data. (A) PCA clustering using smartseq2 data counting only 3’ end to simulate Drop-seq data. While the separation still exists between live and fixed cells, two clusters are not totally separate from each other. (B) PCA clustering using data counting only 5’ end of the transcriptome. The separation between live and fixed cells is clear. (C) PCA clustering including live and fixed cells generated from Drop-seq. Cells from two types are partially merged without strong separation. (D) PCA including fixed and fixed cells with 3 weeks storage. Fixed cells with different storage conditions are clustered together