Fig. 1From: Benchmark of 16S rRNA gene amplicon sequencing using Japanese gut microbiome data from the V1–V2 and V3–V4 primer setsObserved operational taxonomic units (OTUs) and amplicon sequence variants (ASVs) plot at each sequencing depth for V12 (red) and V34 (blue). A qI and B qII. Statistical analysis (Wilcoxon signed-rank test) was performed at a depth of (A: qI) 29,998 and (B: qII) 30,000. Double asterisks indicate statistical significance (p < 0.01)Back to article page