Fig. 7
Matrix metalloproteinases pathway in P compared to NP of day 18 bovine CL and the in vitro effect of IFNT in LGCs. (A) GSEA results showing the activation of matrix metalloproteinase candidate genes enriched in day 18 P compared to NP bovine CL. (B) Heatmap showing the differentially abundant activation of matrix metalloproteinase candidate genes. The heat map was generated by Morpheus - Broad Institute (https://software.broadinstitute.org/morpheus/). Higher and lower levels of transcript accumulation are denoted in red and blue, respectively, and the median level is denoted in white. (C) LGCs (n = 5) were treated with either basal media (control) or roIFNT (1 ng/mL) for 24 h for all except MMP9, which was for 36 h. THBS2, TIMP3, ADAM17, and MMP9 expression was measured by qPCR. Asterisks denote significant differences between roIFNT treatment and controls (***p < 0.001)