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Fig. 5 | BMC Genomics

Fig. 5

From: Functional examination of lncRNAs in allotetraploid Gossypium hirsutum

Fig. 5

A representative NC-lncRNA may regulate its adjacent gene in cis in plants under drought stress. A: Schematic showing the collinear position of XLOC_107738 between Gossypium arboreum (Ga) chr10 and Gossypium hirsutum (Gh) A05. The lower graph shows a stack view of the locus in Gh. Blue box, PCGs; red box, lncRNAs; dashed box, syntenic PCG region. B: Photo showing the phenotype of TRV2::XLOC_107738 and TRV2::00 treated plants (n = 16) before drought treatment, after drought treatment, and after water recovery. Bar = 5 cm. C: Histogram showing the relative expression level of XLOC_107738 in TRV2::XLOC_107738 and TRV2::00 treated plants as quantified by qRT-PCR. Error bar: standard error of the mean. Asterisks indicate significant difference between samples (Student’s t-test, *, p < .05; **, p < .01). R.E.L., relative expression level. D-G: Histograms showing the respective relative expression levels of GhABF1, GhPYL9, GhRD22A, and GhRD22D in TRV2::XLOC_107738 and TRV2::00 treated plants as quantified by qRT-PCR. Error bar: standard error of the mean. Asterisks indicate significant difference between samples (Student’s t-test, *, p < .05; **, p < .01). R.E.L., relative expression level. H: Histogram showing the relative expression of XLOC_107738 and its adjacent PCG Gh_A05G0714 as determined by qRT-PCR. Error bar: standard error of the mean. Asterisks indicate significant difference between samples (Student’s t-test, *, p < .05; **, p < .01). I: Scatterplot showing the linear relationship between expression of XLOC_107738 and that of the adjacent PCG, Gh_A05G0714, under different abiotic stressors. J: Co-expression network of Gh_A05G0714

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