| Low quality | Medium quality | High quality |  |  |  |  | |||
---|---|---|---|---|---|---|---|---|---|---|
Gene | Mean | SEM | Mean | SEM | Mean | SEM | Mean reads | RSQ | RC | P value |
Mitochondrial genes | ||||||||||
 mt-atp8 | 70,396 | 3649 | 105,437 | 10,120 | 144,308 | 19,256 | 117,095 | 0.0355 | 486.1 | 0.3921 |
 mt-co1 | 187,167 | 75,666 | 215,761 | 24,277 | 295,887 | 23,547 | 241,777 | 0.0857 | 1435.7 | 0.0666 |
 mt-cytb | 65,679 | 15,616 | 109,657 | 13,106 | 152,923 | 14,076 | 122,408 | 0.0814 | 776.0 | 0.0426 |
 mt-nd4l | 4920 | 1356 | 6646 | 801 | 10,063 | 1467 | 7733 | 0.0317 | 36.0 | 0.2472 |
 mt-dlp | 9707 | 4788 | 17,151 | 2254 | 23,632 | 4410 | 19,022 | 0.0540 | 132.9 | 0.0839 |
Nuclear genes | ||||||||||
 agfg1-like | 78.3 | 2.0 | 71.8 | 7.2 | 85.9 | 6.2 | 76.9 | 0.0084 | 0.1239 | 0.7307 |
 anxa2 | 53.8 | 8.2 | 73.4 | 8.0 | 80.9 | 9.8 | 75.1 | 0.0145 | 0.1950 | 0.4492 |
 apoc1 | 1209.5 | 793.1 | 2729.3 | 569.8 | 1897.9 | 753.6 | 2376.8 | 0.0022 | 5.5802 | 0.5534 |
 atg16l1 | 52.8 | 2.9 | 70.2 | 7.4 | 76.3 | 7.7 | 71.5 | 0.0146 | 0.1730 | 0.4387 |
 bmp10-like | 13.4 | 1.2 | 39.3 | 4.7 | 35.1 | 3.8 | 36.7 | 0.0130 | 0.1017 | ND |
 ctsz | 9929.1 | 3409.9 | 13,888.0 | 1113.6 | 12,001.9 | 927.1 | 13,065.0 | 0.0010 | 6.6351 | 0.4726 |
 cycB | 14,225.4 | 3878.4 | 14,363.5 | 963.4 | 15,421.9 | 1008.5 | 14,718.0 | 0.0150 | 23.2022 | 0.5360 |
 dcaf11 | 83.8 | 10.0 | 147.6 | 11.4 | 205.1 | 14.6 | 164.3 | 0.1878 | 1.1626 | 0.0035 |
 dglucy | 13.4 | 1.2 | 15.5 | 0.9 | 14.0 | 1.1 | 14.9 | 0.0007 | 0.0046 | ND |
 erich3 | 13.4 | 1.2 | 15.5 | 0.9 | 14.0 | 1.1 | 14.9 | 0.0007 | 0.0046 | ND |
 fbxo5 | 310.2 | 123.7 | 438.7 | 39.5 | 691.7 | 73.1 | 519.2 | 0.1702 | 4.4568 | 0.0120 |
 galnt3 | 354.3 | 16.0 | 230.1 | 20.4 | 254.5 | 19.1 | 244.1 | 0.0219 | −0.5889 | 0.2481 |
 gsh-px | 228.0 | 2.4 | 484.8 | 47.2 | 417.3 | 54.1 | 450.1 | 0.0018 | 0.4046 | 0.4661 |
 gtf3a | 164.5 | 19.9 | 247.2 | 22.1 | 318.0 | 39.6 | 267.6 | 0.0540 | 1.2597 | 0.1150 |
 haus3 | 157.5 | 49.1 | 205.8 | 20.5 | 283.1 | 23.4 | 230.0 | 0.1106 | 1.4526 | 0.0510 |
 hbb | 6945.0 | 2128.3 | 5318.5 | 1111.5 | 2745.4 | 821.5 | 4515.2 | 0.0132 | −23.9143 | 0.2282 |
 ifngr1 | 13.4 | 1.2 | 15.5 | 0.9 | 14.6 | 1.0 | 15.1 | 0.0073 | 0.0146 | ND |
 igf-1 | 13.4 | 1.2 | 23.5 | 2.7 | 23.2 | 2.7 | 22.9 | 0.0657 | 0.1326 | ND |
 igf-2 | 46.9 | 0.8 | 72.9 | 10.1 | 98.2 | 25.0 | 80.4 | 0.0353 | 0.5423 | 0.3252 |
 igf-3 | 13.4 | 1.2 | 29.9 | 3.2 | 26.2 | 3.1 | 27.9 | 0.0146 | 0.0750 | ND |
 igfr1b | 86.0 | 1.4 | 93.0 | 8.1 | 103.4 | 7.1 | 96.2 | 0.0003 | 0.0273 | 0.8610 |
 il17rd | 506.1 | 55.0 | 284.1 | 27.8 | 364.1 | 30.1 | 321.5 | 0.0021 | −0.2719 | 0.5848 |
 impa2 | 511.5 | 186.2 | 1966.8 | 199.4 | 2761.9 | 315.3 | 2171.7 | 0.1689 | 20.1204 | 0.0010 |
 ing3 | 60.8 | 4.4 | 80.0 | 7.2 | 101.8 | 10.5 | 86.5 | 0.0261 | 0.2604 | 0.2269 |
 itga7 | 18.0 | 5.8 | 24.3 | 2.8 | 24.7 | 2.7 | 24.2 | 0.0216 | 0.0772 | ND |
 kmt5b | 70.8 | 7.3 | 61.0 | 5.9 | 70.1 | 6.3 | 64.6 | 0.0008 | 0.0332 | 0.8023 |
 krt18 | 159.7 | 0.8 | 178.3 | 28.9 | 116.5 | 13.6 | 156.4 | 0.0022 | 0.2393 | 0.8316 |
 krt8 | 95.9 | 2.6 | 164.8 | 39.8 | 112.0 | 20.8 | 143.7 | 0.0008 | 0.1936 | 0.5031 |
 lin7b | 135.0 | 28.7 | 399.2 | 44.1 | 493.9 | 60.0 | 419.5 | 0.1175 | 3.3230 | 0.0095 |
 mettl3 | 13.4 | 1.2 | 15.5 | 0.9 | 14.0 | 1.1 | 14.9 | 0.0007 | 0.0046 | ND |
 mr-1 | 316.1 | 97.1 | 327.4 | 20.0 | 355.0 | 24.3 | 336.3 | 0.0000 | −0.0106 | 0.9443 |
 mrpl39-like | 54.3 | 4.5 | 214.6 | 26.9 | 273.2 | 42.6 | 227.3 | 0.0889 | 1.8588 | 0.0126 |
 myo1b | 138.0 | 2.9 | 68.1 | 7.7 | 81.9 | 7.1 | 76.0 | 0.0477 | −0.3429 | 0.1213 |
 nasp | 157.3 | 3.3 | 217.2 | 17.1 | 294.0 | 22.0 | 240.7 | 0.0996 | 1.2183 | 0.0574 |
 npm2 | 13.4 | 1.2 | 16.0 | 0.8 | 14.4 | 1.1 | 15.4 | 0.0001 | −0.0013 | ND |
 ntan1 | 336.7 | 52.6 | 322.3 | 25.8 | 356.8 | 29.8 | 334.7 | 0.0051 | 0.3686 | 0.8127 |
 pde4d | 69.2 | 15.0 | 87.4 | 9.6 | 103.4 | 8.7 | 92.0 | 0.0243 | 0.2860 | 0.2916 |
 pgk1 | 173.4 | 65.0 | 228.0 | 12.1 | 260.1 | 17.6 | 236.5 | 0.0471 | 0.6028 | 0.0465 |
 phb2 | 54.3 | 10.9 | 73.1 | 5.1 | 88.9 | 11.8 | 77.7 | 0.0290 | 0.2436 | 0.2019 |
 psmb9 | 122.7 | 63.9 | 77.6 | 11.0 | 51.5 | 8.0 | 70.7 | 0.0015 | −0.0826 | 0.5031 |
 ptgs2 | 630.3 | 111.9 | 685.1 | 68.2 | 760.7 | 89.5 | 708.4 | 0.0022 | 0.6635 | 0.9055 |
 pyc | 70.7 | 29.5 | 72.8 | 6.2 | 85.2 | 7.4 | 76.9 | 0.0097 | 0.1259 | 0.5615 |
 ran | 44.4 | 3.2 | 41.0 | 6.2 | 39.4 | 4.9 | 40.6 | 0.0106 | 0.1155 | ND |
 rpl22 | 229.6 | 27.9 | 566.2 | 54.1 | 640.3 | 134.1 | 576.2 | 0.0652 | 3.9744 | 0.0130 |
 rpl24 | 654.1 | 0.9 | 1268.8 | 156.9 | 1033.2 | 285.2 | 1160.5 | 0.0065 | 3.0154 | 0.6741 |
 rpl30 | 922.6 | 42.1 | 1505.6 | 154.9 | 1165.1 | 186.5 | 1363.0 | 0.0107 | 3.2904 | 0.5562 |
 rplp1 | 40.4 | 5.7 | 46.5 | 5.7 | 52.3 | 6.0 | 48.2 | 0.0050 | 0.0781 | ND |
 rps9 | 166.8 | 11.0 | 312.3 | 26.5 | 264.3 | 28.1 | 289.4 | 0.0135 | 0.6161 | 0.1591 |
 s100a1 | 115.8 | 22.5 | 154.0 | 15.7 | 192.3 | 15.2 | 165.3 | 0.0643 | 0.7820 | 0.0981 |
 samm50 | 162.2 | 42.9 | 546.5 | 45.3 | 650.0 | 46.0 | 564.3 | 0.1262 | 3.4057 | 0.0024 |
 sec14l2 | 71.6 | 17.4 | 88.6 | 8.0 | 101.8 | 10.5 | 92.3 | 0.0216 | 0.2453 | 0.3916 |
 senp7 | 36.6 | 6.3 | 52.3 | 5.1 | 72.4 | 5.9 | 58.5 | 0.1001 | 0.3488 | 0.0330 |
 ska3 | 89.5 | 20.1 | 187.6 | 17.0 | 253.9 | 21.2 | 205.8 | 0.1664 | 1.5573 | 0.0058 |
 slc7a6os | 184.8 | 14.7 | 337.6 | 23.2 | 393.0 | 29.6 | 349.5 | 0.0732 | 1.3690 | 0.0410 |
 smc6 | 115.2 | 2.4 | 92.9 | 9.3 | 103.0 | 9.4 | 97.4 | 0.0014 | −0.0669 | 0.6010 |
 tfip11 | 40.4 | 5.7 | 80.5 | 6.7 | 113.2 | 13.8 | 89.8 | 0.1357 | 0.6824 | 0.0115 |
 tob1 | 178.8 | 18.3 | 118.6 | 9.8 | 122.8 | 9.9 | 122.8 | 0.0399 | −0.3867 | 0.1576 |
 tubb | 1278.3 | 495.4 | 2522.1 | 174.7 | 3145.6 | 253.3 | 2678.1 | 0.1492 | 15.9537 | 0.0040 |
 uchl1 | 177.0 | 36.0 | 245.8 | 18.8 | 321.2 | 21.5 | 268.4 | 0.1074 | 1.3353 | 0.0348 |
 vasa | 645.4 | 127.0 | 344.1 | 27.9 | 369.0 | 23.6 | 366.2 | 0.0919 | −1.7727 | 0.0507 |