Fig. 1

Multiomic identification of functional HIF1α DNA binding targets in the lens genome. A Duplicate pools of 500,000 primary chick lens cells were cultured with or without the HIF1α activator DMOG (1 mM) for 4 h. Samples were analyzed by CUT&RUN and RNA-seq analysis to identify genome-wide HIF1α-DNA binding complexes and corresponding HIF1α-dependent gene expression changes. Results were compared with ATACseq data [43] to identify the chromatin accessibility of identified HIF1α-DNA binding complexes. Integrated bioinformatics analysis was used to identify the pathways and functions specific for identified HIF1α-targets. B HIF1α is rapidly degraded under normoxic conditions but the presence of DMOG, inhibits prolyl hydroxylase resulting in HIF1α stabilization, DNA binding and transcriptional modulation in lens cells. C-D RT-PCR showing increased levels of the BNIP3L control transcript relative to β-actin in lens cells incubated in the presence of 1 mM DMOG for 4 h. *p < 0.05 t-test n = 3