Fig. 3

HIF1α-DNA binding complexes identified by CUT&RUN cluster in open chromatin regions. A HIF1α-DNA binding complexes were separated into those bound in open chromatin regions and those bound in closed chromatin regions and then sorted into distances away from the transcription start site (0-3kbp, 3-10kbp, 10-100kbp, over 100kbp). The results were compared to what is expected from the computer generated randomly distributed HIF1α-DNA binding complexes. HIF1α-DNA binding complexes were positively associated with open chromatin regions and negatively associated with closed chromatin regions at 0-3kbp, 3-10kbp, 10-100kbp, and over 100kbp distances away from the transcription start site (***p < 1 × 10^− 6) compared to the computer-generated random distribution of HIF1α-DNA binding complexes. B CUT&RUN tracks of HIF1α (red) and IgG negative control (black) within the genomic region of Chr22 encompassing the Gallus gallus gene BNIP3L. The vertical light blue highlight and light blue box indicates a HIF1α-DNA binding complex in the proximal promoter of the gene. The orange track shows the combined chromatin accessibility map representing a combination of the differentiation stage-specific chromatin accessibility tracks obtained from [43] indicating regions of open chromatin in whole chick lens tissue