Fig. 6

CUT&RUN and RNA-seq analysis identifies HIF1α-dependent pathways critical for lens structure, homeostasis, metabolism, and differentiation: A Genes with a HIF1α-DNA binding complex that are significantly downregulated (log2FC < − 0.7, p < 0.05) were input into Enrichr to elucidate significantly associated biological pathways and processes. The top 50 most statistically significant non-redundant pathways are shown. B Genes with a HIF1α-DNA binding complex that are significantly upregulated (log2FC > 0.7, p < 0.05) were input into Enrichr to elucidate significantly associated biological pathways and processes. The top 50 most statistically significant non-redundant pathways are shown. C-E CUT&RUN tracks of HIF1α (red) and IgG negative control (black) within the genomic region encompassing the Gallus gallus genes ENO1 (C), PANK4 (D), and BHLHE40 (E). The vertical light blue highlight and light blue box indicates a HIF1α-DNA binding complex in the proximal promoter of the gene. The orange track shows the combined chromatin accessibility map representing a combination of the differentiation stage-specific chromatin accessibility tracks obtained from [43] indicating regions of open chromatin in whole chick lens tissue