Fig. 3

Reference genes introduce systematic bias despite algorithm based validation. a Relative gene expression (normalized to the mean of group 1 (preterm neonates)) for each candidate reference gene or normalization factor. b Fold-change difference in gene expression of full-term neonates or healthy adults relative to preterm neonates. c Relative gene expression of TLR4 from cord blood of preterm or full-term neonates and peripheral blood of healthy adults is shown non-normalized or normalized to different candidate reference genes or normalization factors. a, c Data is presented as mean ± SD